qPCR

Biology Level pending

What is the "crossing point" or "crossing threshold" of a qPCR reaction?

The relative fluorescence when the second derivative of the qPCR curve is equal to zero. The relative time value when the first derivative of the qPCR curve is equal to zero. The relative fluorescence when the first derivative of the qPCR curve is equal to zero. The relative time value when the second derivative of the qPCR curve is equal to zero.

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